General Cell Based Assays
Molecular Devices cAMP Fluorescent Assay Kit, 384-Well, Bulk, 60 μL
The CatchPoint cAMP Fluorescent Assay Kit is a high-throughput competitive immunoassay for quantifying 3',5'-cyclic adenosine monophosphate (cAMP) and measuring adenylate cyclase activity in biochemical and cell-based assays. The 384-well bulk format supplies reagents for 20 plates (7,680 assay points) and is optimized for fluorescence intensity detection with long signal stability.
- 384-well bulk format, sufficient for 20 plates (7,680 wells)
- Reaction volume 60 μL per well, 50 μL supported for some HTS systems
- Fluorescence intensity detection using Stoplight Red substrate
- Minimum detectable concentration 0.1 nM (6 fmol/well), EC50 approximately 3.4 nM
- Readings stable from 10 minutes up to 24 hours after substrate addition
- Store components at 4°C; kit components stable for six months when stored properly
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-FOXO1 (Ser256) Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Phospho-FOXO1 (Ser256) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc AlphaScreen cAMP Detection Kit, 10,000 Assay Points
AlphaScreen cAMP Detection Kit, 10,000 Assay Points
Biotium XTT Cell Viability Assay Kit (1000 Assays)
The XTT Cell Viability Assay Kit provides a simple method for determining live cell numbers using standard photometric microplate readers. Determination of live cell numbers is often used to assess the rate of cell proliferation and to screen cytotoxic agents. XTT is a tetrazolium derivative. Similar to MTT, XTT measures cell viability based on the activity of mitochondrial enzymes in live cells that reduce XTT and are inactivated shortly after cell death. Unlike MTT, XTT is reduced to a highly water-soluble orange-colored product instead of the insoluble formazan from MTT, thus eliminating the solubilization step required for the MTT assay. The amount of water-soluble product generated from XTT reduction is proportional to the number of living cells in the sample and can be photometrically quantified at 475 nm. Continuous color development permits measurements at multiple time points for extended detection range.
Glass Expansion D-Torch Pro Duo with 2.0mm Quartz Injector
D-Torch complete assembly with 2.0mm ID quartz injector & installation guide. Compatible with Thermo PRO Duo ICP-OES.A patented, precision-engineered, demountable torch, incorporating a ceramic inner tube for greater robustness & demountable quartz outer tube that can be replaced when it fails rather than replacing the entire torch. D-Torch also features an interchangeable injector, allowing installation of a specific injector, whether it be for aqueous (~2.0mm ID), organics (<2.0mm ID), high TDS (>2.0mm ID) or HF (alumina). Compatible with all EMT injectors.The D-Torch is the only design with an optional ceramic outer tube (P/N NC2046972). The quartz outer tube is ideal for most applications while the ceramic outer tube is best for high TDS, organics, or HF matrices. Replacement parts include 31-808-4125, NC2046973, NC2046972, 50-169-4654, 31-808-2707, 31-808-2895, 31-808-2897, 70-803-1520.Glass Expansion is ISO 9001:2015 certified by AB Certification, No. AU4501-9001
Eurofins Discoverx PathHunter Mouse CRHR1 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse CRHR1 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CRHR1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cell Biolabs Inc NAD+/NADH Assay Kit (Colorimetric), 100 assays
Cell Biolabs’ NAD+/NADH Assay Kit (Colorimetric) is a simple assay that measures NAD+ and NADH present in biological samples such as cell lysates or tissue extracts in a 96-well microtiter plate format. The kit is specific for NAD+, NADH, and their ratio. The kit will not detect NADP+ or NADPH. The total NAD+/NADH concentrations of unknown samples are determined by comparison with a known NAD+ standard. Determination of both NAD+ and NADH requires two separate samples for quantification. NAD+ and NADH do not need to be purified from samples, but rather can be extracted individually with a simple acid or base treatment prior to performing the assay. The kit has a detection sensitivity limit of approximately 4 nM NAD+. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, NAD+ standards and unknown samples.
Eurofins Discoverx PathHunter eXpress GPR32 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0321E2ACP2L)
The PathHunter GPR32 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR32 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR32 and EA-tagged β-Arrestin-2. Activation of GPR32 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress P2RY6 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0500E2CP19M)
The PathHunter P2RY6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. UTP) based activation of P2RY6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY6 and EA-tagged β-Arrestin-2. Activation of P2RY6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NMBR Total Internalization Assay, 2pl Kit. (93-0939E3CP5M)
The PathHunter NMBR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of NMBR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged NMBR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the NMBR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-SMAD3 (Ser423/425) Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Phospho-SMAD3 (Ser423/425) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-H2AX (Ser139) Detection Kit, 500 Assay Points
HTRF Human Phospho-H2AX (Ser139) Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-FAK (Tyr397) Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Phospho-FAK (Tyr397) Detection Kit, 10,000 Assay Points
Meso Scale Discovery U-PLEX Mouse IL-16 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-16 Assay is a multiplex immunoassay kit that enables simultaneous quantification of mouse interleukin 16 (IL-16) in serum plasma and cell culture supernatants This electrochemiluminescent assay utilizes MSD s proprietary SECTOR Imager and provides high sensitivity a wide dynamic range and requires low sample volume The kit includes a 96-well plate pre-coated with capture antibodies detection antibodies calibrators and controls Results are obtained in as little as 3 5 hours This assay allows for efficient reproducible analysis of IL-16 levels relevant to immunology and inflammation research